asyn  (Santa Cruz Biotechnology)

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: The QuantiGene Plex assay that utilized upstream and downstream probe sets for rat Snca showed specific signals (designated as Normalized MFI) only in samples from WT rats but not in samples from aSyn KO animals in the cortex (A), hippocampus (B), substantia nigra (C) and cerebellum (D). Data were analyzed using ordinary two-way ANOVA with genotype and age as independent factors. If the genotype × age effect was significant, pairwise comparisons within the same age group were done by the Holm–Šídák’s multiple comparisons test as follows: **** P < 0.0001. If the factor interaction effect was not significant, main genotype effect was illustrated as follows: #### P < 0.0001.

Article Snippet: The sections were then incubated overnight at room temperature with rabbit polyclonal antibody against total aSyn (#2628S, Cell Signaling Technology, Beverly, MA, USA) 1:200 dilution in 1% NGS in 1x TBST.

Techniques: Plex Assay

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: (A) Relative expression of rat aSyn in the cortex of aSyn KO and associated WT rats. (B) Western blot image of total aSyn and actin western blot in cortex of aSyn KO and WT rats. (C) Relative expression of human aSyn in the cortex of aSyn A53T KI and associated WT rats. (D) Western blot image of total aSyn, triton-soluble aSyn, and triton-insoluble aSyn in cortex of aSyn A53T KI and WT rats. Horizontal bars indicate the median value. Data were analyzed using two-way repeated measures ANOVA with genotype and age as independent factors. Main genotype effect was illustrated as follows: # P < 0.05.

Article Snippet: The sections were then incubated overnight at room temperature with rabbit polyclonal antibody against total aSyn (#2628S, Cell Signaling Technology, Beverly, MA, USA) 1:200 dilution in 1% NGS in 1x TBST.

Techniques: Expressing, Western Blot

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: (A, B) Stool counts in female and male aSyn A53T KI and WT rats of different ages. (C, D) Bead expulsion times in female and male aSyn A53T KI and WT rats of different ages. Data in A–D are presented as box-whisker plots. Data were analyzed using ordinary two-way ANOVA with genotype and age as independent factors (Stool count averaged over D1 and D2).

Article Snippet: The sections were then incubated overnight at room temperature with rabbit polyclonal antibody against total aSyn (#2628S, Cell Signaling Technology, Beverly, MA, USA) 1:200 dilution in 1% NGS in 1x TBST.

Techniques: Whisker Assay

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: Striatal levels of dopamine (A, B), DOPAC (C, D), and HVA (E, F) were determined using HPLC in samples of 4- and 18-month-old aSyn A53T KI rats and 6- as well as 12-month-old aSyn KO rats and associated WT littermates. Data were analyzed using Mixed-effects model (REML) with genotype and age as independent factors. Results are indicated as follows: *** P < 0.001 age effect.

Article Snippet: The sections were then incubated overnight at room temperature with rabbit polyclonal antibody against total aSyn (#2628S, Cell Signaling Technology, Beverly, MA, USA) 1:200 dilution in 1% NGS in 1x TBST.

Techniques:

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: (A) Box-whisker plots of TH + cell numbers in the SNpc of aSyn A53T KI and WT rats. (B) Images of midbrain sections stained for TH to visualize dopaminergic neurons in the SNpc.

Article Snippet: The sections were then incubated overnight at room temperature with rabbit polyclonal antibody against total aSyn (#2628S, Cell Signaling Technology, Beverly, MA, USA) 1:200 dilution in 1% NGS in 1x TBST.

Techniques: Whisker Assay, Staining

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: (A-C) Representative images of staining for human/rat aSyn in the (A) midbrain, (B) colon, and (C) duodenum of WT and aSyn A53T KI rats. stained for human/rat aSyn. (D-F) Representative images of staining for pS129 aSyn in the (D) SNpc at 20x, (E) colon, and (F) duodenum. Colon and duodenum display high background fluorescence for pS129 aSyn.

Article Snippet: The sections were then incubated overnight at room temperature with rabbit polyclonal antibody against total aSyn (#2628S, Cell Signaling Technology, Beverly, MA, USA) 1:200 dilution in 1% NGS in 1x TBST.

Techniques: Staining, Fluorescence

Journal: PLOS One

Article Title: Expression of human A53T alpha-synuclein without endogenous rat alpha-synuclein fails to elicit Parkinson’s disease-related phenotypes in a novel humanized rat model

doi: 10.1371/journal.pone.0329823

Figure Lengend Snippet: (A) Representative images of staining for pS202/T205 tau in the cortex of WT and aSyn A53T KI mice. (B) Representative images of staining for Iba1-positive microglia in the SNpc at 10x and 20x magnification. (C) Representative images of GFAP-positive astrocytes in the brain.

Article Snippet: The sections were then incubated overnight at room temperature with rabbit polyclonal antibody against total aSyn (#2628S, Cell Signaling Technology, Beverly, MA, USA) 1:200 dilution in 1% NGS in 1x TBST.

Techniques: Staining